1.0  INTENDED USE

   A simple and efficient extraction and purification procedure for 3-amino-2-oxazolidone(AOZ)was developed by means of the immunoaffinity column (IAC-AOZ) as a cleanup tool．AOZ content in Feeds,Aquatic Products and Animal Derived Food are cleaned up by IAC and determined by HPLC or LC/MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring AOZ

2.0 PRINCIPLE

   Samples are prepared by mixing with an extraction solution, blending and filtering.  The extract is then applied to the AOZ immunoaffinity column bound with specific antibodies to AOZ．At this stage, the AOZ binds to the antibody on the column.  The column is then washed with water to rid the immunoaffinity column of impurities.  By passing methanol through the column, the AOZ is removed from the antibody.  This methanol solution can then be injected into HPLC or LC/MS/MS system.