C/N: EE 115     Sensitivity:0.1 ppb    Test Range: 0.1~20.0 ppb

1. BACKGROUND
    ß-Agonists, such as clenbuterol or salbutamol, are synthetic derivatives of the naturally occurring catecholamines. It has been known that ß-agonists are suitable for use as performance improvers within the field of livestock production; in particular, the meat/fat ratio in fattened animals can be improved or the growth may be accelerated. However, such compounds have not been approved in the EU for use as fattening adjuvants. In addition to lipolytic and anabolic effects, ß-agonists have relaxing effects on non-striated musculature. Thus they can be used as antiasthmatic and tocolytic agents. It is possible that ß-agonists residues, after use in illegal practice, may lead to a risk for consumers. Therefore it exists a prohibition of ß-agonists use in food production.

2. PURPOSE
     This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the clenbuterol concentration in the sample.

3. KIT CONTENTS
1)Microtiter plate (8 wells×12) precoated with antigen.
2)7×Standard solutions (1ml/each):0ng/ml, 0.1ng/ml, 0.3ng/ml, 1.0ng/ml, 3.0ng/ml, 9.0ng/ml, 27.0ng/ml
Controls provided: 0ppb, 0.1ppb, 0.3ppb, 1.0ppb, 3.0ppb, 9.0ppb and 27.0ppb 
3)Antibody (100×)                   (0.2ml)
4)Enzyme Conjugate(100×)            (0.2ml)
5)Washing buffer(10×)                (50ml)
6)Antibody and Enzyme diluent buffer   (30ml)
7)Diluent buffer A                   (20ml)
8)Diluent buffer B                   (40ml)
9)Diluent buffer C                   (40ml)
10)TMB                              (18ml)
11)Stop solution                     (7ml)
12)Product Manual