C/N: EE 205    Sensitivity: 0.5 ppb    Test Range: 0.5~50.0 ppb

1. BACKGROUND
    The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of  its estrogenic properties, zearalenone may induce  fertility  disorders  in  animals with  clinical  signs  of  hyperestrogenism-anaspect of a disease which although reported mainly in hogs, is described in other species such as cow,  horse  and  sheep,  too.  The  potential  health  risk  for man induced  by  this mycotoxin, which  is  taken  up with  foods  of  vegetable  or  animal origin, is extensively discussed.

2. PURPOSE
    This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Zearalenone residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the clenbuterol concentration in the sample.

3. KIT CONTENTS
1)Microtiter plate (8 wells×12) precoated with antigen.
2)6×Zearalenone standard solutions (1ml/each):0ng/ml, 0.5ng/ml, 2.0ng/ml, 5.0ng/ml, 15.0ng/ml, 50.0ng/ml
Controls provided: 0ppb, 0.5ppb, 2.0ppb, 5.0ppb, 15.0ppb and 50.0ppb
3)Antiboby (100×): Zearalenone        (0.2ml)
4)Enzyme Conjugate(100×)            (0.2ml)
5)Washing buffer(10×)                (50ml)
6)Antibody and Enzyme diluent buffer   (30ml)
7)Diluent buffer                     (50ml)
8)TMB                               (13ml)
9)Stop solution                      (7ml)
10)Product Manual