C/N:EE 206    Sensitivity: 0.01 ppm    Test Range: 0.01~0.2 ppm

1. BACKGROUND
    Deoxynivalenol (DON, Vomitoxin) is a damaging toxin produced by the fungus Fusarium graminearum in the heads of small grains. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and maize, and less often in rice, sorghum, and triticale. The occurrence of deoxynivalenol is associated primarily with Fusarium graminearum (Gibberella zeae) and F. culmorum, both of which are important plant pathogens which cause fusarium head blight in wheat and gibberella or fusarium ear blight in maize. Large amounts of grain with deoxynivalenol would have to be consumed to pose a health risk to humans. The U.S. Food and Drug Administration has established a level of 1 ppm (parts per million) restriction of deoxynivalenol.

2. PURPOSE
    This handbook establishes procedures for determining deoxynivalenol(DON) in corn, grains and wheat. The test is an indirect competitive ELISA that provides exact concentrations in parts per billion (ppb). Free toxin in the sample and controls competes with enzyme-labeled toxin (conjugate) for the antibody binding sites. After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color. A stop solution is then added which changes the color from blue to yellow. The intensity of the color is inversely proportional to the concentration of deoxynivalenol in the samples and standards.

3. KIT CONTENTS
1)Microtiter plate (8wells×12): precoated with antigens to mouse IgG.
2)6×DON standard solutions (1ml/each): 0ng/ml, 10ng/ml, 50ng/ml, 200ng/ml, 1000ng/ml, 5000ng/ml
3)Anti- DON antibody (20×): 0.2ml
4)Enzyme Second Antibody (80×): 0.4ml
5)Washing buffer(10×): 50ml
6)Antibody and Enzyme diluents buffer: 30ml
7)Diluent buffer: 50ml
8)TMB: 17ml
9)Stop solution: 7ml
10)Product Manual