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Clover Technology Group, Inc. is proud to present our iElisa Sulfamethazine Kit, a high-performance enzyme-linked immunosorbent assay designed for the precise and quantitative determination of sulfamethazine residues in grain and processed grain products. This kit is an essential tool for food safety monitoring, quality control, and regulatory compliance.
Sulfonamides are a class of broad-spectrum antimicrobial agents widely used in veterinary medicine and as feed additives, primarily for growth promotion and disease prevention in calves and pigs. They are often combined with dihydrofolate reductase inhibitors (such as trimethoprim, tetroxoprim, or pyrimethamine) to treat intestinal infections, mastitis, pneumonia, and other systemic diseases in food-producing animals.
While effective for their intended purposes, the widespread use of sulfonamides raises significant food safety concerns. Residues of these compounds can persist in animal-derived foods such as meat, milk, and eggs. Among the sulfonamide family, sulfamethazine is of particular concern due to its potential carcinogenic properties, posing a threat to human health upon chronic exposure through the food chain.
To protect consumer health, regulatory bodies worldwide have established strict maximum residue limits (MRLs). According to European Union regulations, the MRL for all substances within the sulfonamide group is set at 100 parts per billion (ppb) in muscle, fat, liver, kidney, and milk. The iElisa Sulfamethazine Kit is specifically developed to help food producers, laboratories, and regulatory agencies enforce these limits effectively and ensure food safety.
The iElisa Sulfamethazine Kit is a competitive direct ELISA designed for simplicity, speed, and accuracy. It provides quantitative results in ppb (ng/mL), enabling users to determine if sulfamethazine levels in their samples comply with legal thresholds. Key features of our kit include:
High Sensitivity and Specificity: The kit offers a detection range of 0.5 to 50.0 ppb, allowing for the identification of trace contamination levels well below the regulatory MRL of 100 ppb. The antibodies used are highly specific to sulfamethazine, minimizing cross-reactivity with other compounds.
Streamlined Procedure: The competitive ELISA format is straightforward to perform, with a short incubation time and clear colorimetric readout, making it suitable for routine screening.
Comprehensive Kit Contents: The iElisa Sulfamethazine Kit supplies all necessary reagents for a complete assay, simplifying laboratory workflow and reducing the need for additional materials.
The kit includes the following components:
Microtiter Plate: One plate with 8 wells × 12 strips, pre-coated with antibodies to mouse IgG.
Sulfamethazine Standards: Six standard solutions (1 mL each) at concentrations of 0, 0.5, 2.0, 5.0, 20.0, and 50.0 ng/mL (ppb).
Antibody Solution (100×): 0.2 mL of anti-sulfamethazine antibody.
Enzyme Conjugate (100×): 0.2 mL of enzyme-labeled sulfamethazine conjugate.
Washing Buffer (10×): 50 mL of concentrated buffer for plate washing.
Antibody and Enzyme Diluent Buffer: 30 mL for preparing working solutions of the antibody and conjugate.
Diluent Buffers A, B, and C: 40 mL each, for sample preparation and dilution.
TMB Substrate: 18 mL of 3,3',5,5'-tetramethylbenzidine solution for color development.
Stop Solution: 7 mL of stopping reagent to halt the enzymatic reaction.
The iElisa Sulfamethazine Kit operates on the principle of competitive direct ELISA. The assay procedure can be summarized as follows:
Sample Preparation: Grain or processed grain samples are extracted and prepared according to the instructions provided in the product manual.
Competitive Binding: The sample or standard is added to the microtiter wells along with the sulfamethazine-specific antibody. In a separate step, the enzyme conjugate is added. Free sulfamethazine in the sample (or standards) competes with the enzyme-labeled conjugate for binding to the limited number of antibody sites pre-coated on the wells. The amount of antibody-bound conjugate is inversely proportional to the concentration of sulfamethazine in the sample.
Washing: Unbound materials are removed through a series of washing steps using the provided washing buffer.
Color Development: The TMB substrate is added to the wells. The enzyme on the bound conjugate catalyzes a reaction with the substrate, producing a blue color.
Stopping and Measurement: The stop solution is added to halt the reaction, turning the solution yellow. The color intensity (optical density) is measured using a microplate reader at 450 nm.
Quantification: A standard curve is plotted using the known concentrations of the standards. The concentration of sulfamethazine in the sample is then determined by comparing its optical density to this standard curve.
The iElisa Sulfamethazine Kit is an ideal solution for:
Food and Feed Manufacturers: To conduct in-house quality control and ensure raw materials and finished products meet safety standards.
Testing Laboratories: For routine screening of grain and processed grain products destined for animal feed or human consumption.
Regulatory and Quarantine Agencies: To enforce food safety regulations and monitor for veterinary drug residues in the food supply chain.
Research Institutions: For studies related to food safety, environmental monitoring, and analytical method development.
With the iElisa Sulfamethazine Kit, Clover Technology Group, Inc. reaffirms its commitment to providing innovative, reliable, and user-friendly solutions for global food safety challenges. By enabling the accurate detection of sulfamethazine residues, our kit empowers stakeholders across the food production and regulatory sectors to uphold the highest standards of public health protection.
For more information on the iElisa Sulfamethazine Kit or to place an order, please visit our website or contact our sales team. Choose Clover Technology Group, Inc. – your trusted partner in food safety diagnostics.