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In the demanding fields of pharmaceutical quality control, food safety testing, and biomedical research, analyzing specific compounds like low molecular weight heparins (LMWHs), glycosaminoglycans (GAGs), or heparin-binding proteins often presents a significant challenge. These target molecules are typically buried within complex biological matrices—such as plasma, tissue homogenates, or processed products—that contain a high concentration of interfering substances like proteins, salts, and other biomolecules. Conventional sample preparation methods often fall short, leading to poor recovery, low sensitivity, and unreliable results that can compromise the entire analytical workflow, from HPLC to mass spectrometry.
Clover Technology Group, Inc. addresses this critical bottleneck with its high-performanceHeparin Immunoaffinity Columns (IAC). These columns are not simple filters; they are sophisticatedbio-specific purification tools. By leveraging the powerful and specific interaction between heparin and its binding proteins/antibodies immobilized on a robust chromatography medium, our columns achieve unparalleledselectivity, efficiency, and reproducibilityin sample cleanup. They are designed to isolate and concentrate your target analyte in a single step, transforming "dirty" samples into "analysis-ready" eluates.
The core of our technology lies inimmunoaffinity chromatography. A highly specific anti-heparin antibody (or a high-affinity heparin-binding protein) is covalently and stably immobilized onto an agarose or Sepharose gel within a user-friendly column. When a prepared sample is passed through the column:
Specific Capture:Heparin molecules (or other ligands with affinity) in the sample are selectively and tightly bound by the immobilized capture agent.
Stringent Washing:All non-specifically bound contaminants—proteins, pigments, lipids—are thoroughly washed away under optimized buffer conditions, dramatically reducing background noise.
Pure Elution:The purified target heparin (or binding protein) is then gently released using a mild, specific elution buffer, resulting in a highly concentrated and clean sample fraction.
| Feature | Technical Advantage | Benefit to Your Laboratory |
|---|---|---|
| High Specificity & Capacity | Covalently immobilized, high-affinity ligands with optimized binding capacity. | Maximizes target capture (e.g., LMWHs, GAGs, specific proteins) while rejecting >99% of impurities. |
| Excellent Reproducibility | Rigorously controlled manufacturing ensures consistent ligand density and column performance. | Achieves reliable recovery rates and results across multiple batches and users. |
| Robust & Durable | Stable ligand coupling and column design allow for multiple regeneration and re-use cycles. | Significantly reduces cost per sample and increases workflow efficiency. |
| Broad Compatibility | Compatible with various sample matrices (plasma, urine, cell lysates, feed, tissue). | A versatile tool for R&D, QC, and diagnostic assay development across different applications. |
| Ready-to-Use Format | Supplied pre-packed, equilibrated, and with a detailed protocol. | Saves time, simplifies operation, and minimizes user-to-user variability. |
Pharmaceutical Analysis:Purification and quantification oflow molecular weight heparins (e.g., enoxaparin, dalteparin)and heparin contaminants (like OSCS) for drug safety and potency testing, aligning withUSP and EP monographrequirements.
Food Safety & Regulatory Testing:Efficient cleanup for the detection ofheparin and related glycosaminoglycan residuesin animal-derived food products (e.g., meat, dairy) to ensure regulatory compliance.
Biomedical & Proteomics Research:Isolation ofheparin-binding proteins(growth factors, cytokines, enzymes) from complex biological fluids for downstream analysis (ELISA, Western Blot, Mass Spec).
Clinical Research Sample Prep:Purification of heparin from patient samples for pharmacokinetic studies or biomarker discovery.
We provide:
Detailed validation data for specific applications.
Customization options for unique project requirements.
Expert technical consultation to optimize your sample preparation protocol.