iElisa Melamine Kit: Reliable, High-Throughput Screening for Melamine Residues in Food and Feed

2026-03-10

Food safety scandals involving melamine adulteration in milk, infant formula, and pet food have underscored the critical need for robust, sensitive, and efficient screening methods. Melamine, a nitrogen-rich industrial chemical, has been illegally added to food and feed to falsely elevate apparent protein content in standard tests, posing serious health risks to consumers and animals alike.

TheiElisa Melamine KitfromClover Technology Group, Inc.provides a reliable, high-throughput enzyme-linked immunosorbent assay (ELISA) solution for the quantitative detection of melamine residues in a variety of matrices. Designed for laboratories seeking accuracy, efficiency, and regulatory compliance, this kit enables rapid screening of large sample batches with confidence.

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The Importance of Melamine Testing

Melamine contamination, whether from intentional adulteration or migration from packaging materials, requires vigilant monitoring across the food supply chain:

Dairy Products:Milk, yogurt, cheese, and especially infant formula are high-risk products requiring stringent testing.
Pet Food:The 2007 pet food recalls highlighted the deadly consequences of melamine adulteration in animal feed.
Animal Feed:Contaminated feed not only harms livestock but can also lead to residues in meat, milk, and eggs.
Processed Foods:Bakery products, confectionery, and protein supplements may be vulnerable to adulteration.

Regulatory bodies worldwide, including the FDA, EFSA, and China's NHFPC, have established strict tolerance levels for melamine in food and feed, making reliable testing methods essential for compliance and consumer safety.

Introducing the iElisa Melamine Kit: Science Meets Simplicity

Our iElisa Melamine Kit is built on a competitive ELISA platform, optimized for sensitivity, specificity, and ease of use in routine laboratory settings.

Core Principle:The assay utilizes highly specific antibodies that recognize melamine. Melamine in the sample competes with melamine-protein conjugate coated on the microplate for antibody binding sites. After washing away unbound materials, an enzyme-catalyzed color reaction produces a signal inversely proportional to the melamine concentration in the sample. The intensity of the color is measured using a standard microplate reader, and concentrations are calculated against a standard curve.

Key Features & Advantages

Feature	Benefit
High Sensitivity	Detection limits as low as2 ppb(μg/L) — meets and exceeds global regulatory requirements
Excellent Specificity	Minimal cross-reactivity with structurally similar compounds (e.g., cyanuric acid, ammeline) ensures accurate results
High Throughput	96-well microplate format enables simultaneous screening of up to 40+ samples in duplicate
Broad Sample Compatibility	Validated for milk, milk powder, infant formula, pet food, animal feed, and more
Simple Protocol	Clear, step-by-step procedure with minimal hands-on time — no specialized training required
Cost-Effective	Dramatically reduces per-sample cost compared to LC-MS/MS, ideal for routine screening
Full Kit Includes	Pre-coated microplate, standards, conjugate, substrate, stop solution, and detailed protocol

Simple Workflow: From Sample to Result in Under 2 Hours

Sample Preparation:Homogenize and extract melamine from the sample matrix using the provided extraction buffer. A simple dilution step prepares the extract for analysis.
Assay Setup:Add standards and prepared samples to the antibody-coated microplate wells, followed by enzyme conjugate. Incubate.
Washing:Remove unbound materials with the provided wash buffer.
Color Development:Add substrate solution — a blue color develops in wells containing melamine.
Stop & Read:Add stop solution, turning the color yellow. Measure absorbance at 450 nm using a microplate reader.
Data Analysis:Calculate melamine concentrations by comparing sample absorbance to the standard curve.

Applications Across the Food & Feed Industry

Dairy Processors:Routine screening of incoming raw milk and finished dairy products
Infant Formula Manufacturers:Ensuring the highest safety standards for vulnerable consumers
Pet Food Companies:Verifying ingredient integrity and final product safety
Feed Mills:Monitoring raw materials and finished feed for livestock
Regulatory Laboratories:High-throughput surveillance and compliance testing
Third-Party Testing Labs:Offering melamine screening services to clients

Performance That Inspires Confidence

The iElisa Melamine Kit has been rigorously validated against LC-MS/MS, the gold standard reference method, demonstrating excellent correlation (R² > 0.98) across relevant concentration ranges. Key performance metrics include:

Limit of Detection (LOD):2 ppb
Limit of Quantitation (LOQ):5 ppb
Recovery Rate:80-120% across validated matrices
Coefficient of Variation (CV):<10% intra-assay and inter-assay

Why Choose CWVK's iElisa Solutions?

At Clover Technology Group, Inc., we understand the demands of modern food safety laboratories. Our iElisa product line represents the convergence of robust immunodiagnostic technology with practical, user-centric design. Each kit undergoes stringent quality control to ensure batch-to-batch consistency and reliable performance.

Benefits of choosing CWVK:

Technical Support:Our team of application specialists is available to assist with protocol optimization and troubleshooting.
Comprehensive Validation:Kits are validated on real-world samples, not just buffer systems.
Regulatory Alignment:Detection limits are designed to meet or exceed major global regulatory standards.
Continuous Innovation:We regularly update our products based on customer feedback and emerging testing needs.

Safeguard your brand, protect consumers, and ensure regulatory compliance with the iElisa Melamine Kit — where accuracy meets efficiency.

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