C/N：IAC 306B

1.0 INTENDED USE

A simple and efficient extraction and purification procedure for5-morpholine-methyl-3-amino-

2-oxazolidinone (AMOZ)was developed by means of the immunoaffinity column (IAC-AMOZ) as a cleanup tool．AMOZ content in Feeds,Aquatic Products and Animal Derived Foodare cleaned up by IAC and determined by HPLC or LC/MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring AMOZ

2.0 PRINCIPLE

Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the AMOZ immunoaffinity column bound with specific antibodies to AMOZ．At this stage, the AMOZ binds to the antibody on the column. The column is then washed with water to rid the immunoaffinity column of impurities. By passing methanol through the column, the AMOZ is removed from the antibody. This methanol solution can then be injected into HPLC or LC/MS/MS system.