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1.0 PRINCIPLE
Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Deoxynivalenol immunoaffinity column bound with specific antibodies to Deoxynivalenol.At this stage, the Deoxynivalenol binds to the antibody on the column. The column is then washed with water to remove the impurities. By passing methanol through the column, the Deoxynivalenol is removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.
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2.0 INTENDED USE
A simple and efficient extraction and purification procedure for Deoxynivalenol was developed by means of the immunoaffinity column (IAC-SEP ® DON) as a cleanup tool.Deoxynivalenol content in corn, wheat, oats, barley, rice, and other grains are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast, simple, safe and highly accurate method for quantitatively measuring DON.
3.0 APPLICATIONS
Grain, peanuts and peanuts products, Feed, Soy sauce, Vinegar, Liquor, etc.
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4.0 ORDERING INFORMATION
Cat. No. Description
IAC 111 25 IAC/box
5.0 SPECIFICATIONS
Format |
3 ml |
Specificity |
Deoxynivalenol |
Column capacity |
2000ng |
Storage Conditions |
2 - 8 °C |
Shelf Life |
18 Months |
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