1.0 PRINCIPLE

Samples are prepared by mixing with an extraction solution(or just centrifuging), blending and filtering.The extract is then applied to the Aflatoxin M1 immunoaffinity column bound with specific antibodies to Aflatoxin M1．At this stage, the Aflatoxin M1 bind to the antibody on the column. The column is then washed with water to remove the impurities. By passing methanol through the column, the Aflatoxin M1 are removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.

2.0 INTENDED USE

A simple and efficient extraction and purification procedure for Aflatoxins was developed by means of the immunoaffinity column (IAC - SEP ® AFLA-M1) as a cleanup tool．This method do not use toxic solvents such as chloroform and methylene chloride. Aflatoxin M1 content in milk, fermented milk, powder , infant formula powder, cheese and cream samples are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring aflatoxin M1.

3.0 APPLICATIONS

Milk, Powdered Milk , Fermented milk, Cream, Cheese, etc.

4.0 ORDERING INFORMATION

Cat. No. Description

IAC 102 25 IAC/box

5.0 SPECIFICATIONS