1.0 PRINCIPLE

Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Aflatoxin immunoaffinity column bound with specific antibodies to Aflatoxin．At this stage, the Aflatoxin binds to the antibody on the column. The column is then washed with water to remove the impurities. By passing methanol through the column, the Aflatoxin is removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.

2.0 INTENDED USE

A simple and efficient extraction and purification procedure for Aflatoxin was developed by means of the immunoaffinity column (IAC-SEP®AFLA-B1) as a cleanup tool．Aflatoxin content in grain, food, feed, nuts, peanuts, soy sauce, vinegar, chili, pepper, medicinal herbs and wine samples are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast, simple, safe and highly accurate method for quantitatively measuring aflatoxin-B1

3.0 APPLICATIONS

Rice, Corn, Wheat, Nuts, Peanuts & Peanuts products, Vegetable oil,

Soy sauce, Vinegar, Feed, Spices, etc.

4.0 ORDERING INFORMATION

Cat. No. Description

IAC 101 25 IAC/box

5.0 Specifications