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IAC-CZ

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INTRODUCTION

1.0 PRINCIPLE

Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Zeranols and Chloram- phenicol immunoaffinity column bound with specific antibodies to Zeranols and Chloramphenicol .At this stage, the Zeranols and Chloramphenicol binds to the antibody on the column. The column is then washed with water to rid the immunoaffinity column of impurities. By passing methanol through the column, the Zeranols and Chloramphenicol is removed from the antibody. This methanol solution can then be injected into HPLC or LC/MS/MS system.


2.0INTENDED USE

A simple and efficient extraction and purification

procedure for Zeranols andChloramphenicolwas developed

by means of the immunoaffinity column (IAC-CZ) as a

cleanup tool.Zeranols andChloramphenicolcontent in Feeds,Aquatic Products and Animal Derived Food are cleaned up by

IAC and determined by HPLC or LC/MS/MS. It is a fast,simple

, safe and highly accurate method for quantitatively

measuring Zeranols (6 analogs) andChloramphenicol

Chloramphenicoland 6 kinds of Zeranols

α-zearalanol/zeranol

α-zearalenol

β-zearalenol

β-zearalanol/β-zeranol

zearalenone

zearalanone

Chloramphenicol



3.0 APPLICATIONS

Aquatic Products and Animal Derived Food, Urine,Feeds. etc.


4.0 ORDERING INFORMATION

Cat. No. Description

IAC 311C 25 IAC/box


5.0 Specifications

Format

3ml

Specificity

Zeranols/Chloramphenicol

Column capacity

Zeranols≥500ng

CAP≥200ng

Storage Conditions

2 - 8 °C

Shelf Life

18 Months

Recovery

≥80%


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