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1.0 PRINCIPLE
Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Zeranols and Chloram- phenicol immunoaffinity column bound with specific antibodies to Zeranols and Chloramphenicol .At this stage, the Zeranols and Chloramphenicol binds to the antibody on the column. The column is then washed with water to rid the immunoaffinity column of impurities. By passing methanol through the column, the Zeranols and Chloramphenicol is removed from the antibody. This methanol solution can then be injected into HPLC or LC/MS/MS system.
2.0INTENDED USE
A simple and efficient extraction and purification
procedure for Zeranols andChloramphenicolwas developed
by means of the immunoaffinity column (IAC-CZ) as a
cleanup tool.Zeranols andChloramphenicolcontent in Feeds,Aquatic Products and Animal Derived Food are cleaned up by
IAC and determined by HPLC or LC/MS/MS. It is a fast,simple
, safe and highly accurate method for quantitatively
measuring Zeranols (6 analogs) andChloramphenicol
Chloramphenicoland 6 kinds of Zeranols
α-zearalanol/zeranol |
α-zearalenol |
β-zearalenol |
β-zearalanol/β-zeranol |
zearalenone |
zearalanone |
3.0 APPLICATIONS
Aquatic Products and Animal Derived Food, Urine,Feeds. etc.
4.0 ORDERING INFORMATION
Cat. No. Description
IAC 311C 25 IAC/box
5.0 Specifications
Format |
3ml |
Specificity |
Zeranols/Chloramphenicol |
Column capacity |
Zeranols≥500ng CAP≥200ng |
Storage Conditions |
2 - 8 °C |
Shelf Life |
18 Months |
Recovery |
≥80% |
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