1.0 PRINCIPLE

Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Phenobarbital immunoaffinity column bound with specific antibodies to Phenobarbital．At this stage, the Phenobarbital binds to the antibody on the column. The column is then washed with water to remove the impurities in immunoaffinity column. By passing methanol through the column, the Phenobarbital is removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.

2.0INTENDED USE

A simple and efficient extraction and purification procedure for Phenobarbital was developed by means of the immunoaffinity column (IAC-PB) as a cleanup tool．Phenobarbital content in Feeds are cleaned up by IAC and determined by HPLC or LC-MS. It is a fast, simple, safe and highly accurate method for quantitatively measuring PB.

3.0 APPLICATIONS

Feed. etc.

4.0 ORDERING INFORMATION

Cat. No. Description

IAC 308 25 IAC/box

5.0 Specifications