1.0 PRINCIPLE

Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Chloramphenicol immunoaffinity column bound with specific antibodies to Chloramphenicol．At this stage, the Chloramphenicol binds to the antibody on the column. The column is then washed with water to remove the impurities in immunoaffinity column. By passing methanol through the column, the Chloramphenicol is removed from the antibody. This methanol solution can then be injected into HPLC or LC/MS/MS/MS system.

2.0 INTENDED USE

A simple and efficient extraction and purification procedure for Chloramphenicol was developed by means of the immunoaffinity column (IAC-CAP) as a cleanup tool．Chloramphenicol content in Feeds,Honey,Milk,Urine,Aquatic Products and Animal Derived Food are cleaned up by IAC and determined by HPLC or LC/MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring CAP.

3.0APPLICATIONS

Aquatic Products ,Animal Derived Food, milk and powdered milk. etc.

4.0 ORDERING INFORMATION

Cat. No. Description

IAC 309 25 IAC/box

5.0 SPECIFICATIONS