C/N：EE2051

1. BACKGROUND

iELISA® tetracycline Assay is a direct competitive enzyme-linked immunosorbent assay (ELISA) that determines a quantitative level of tetracycline and is intended for use in meet, milk, feed and eggs.

2. PURPOSE

The basis of the assay is the antigen-antibody reaction.The extracted sample or control standards and enzyme-antibody are mixed and added to the antigen-coated microwell.Then Free tetracycline in samples and standards are allowed to compete with tetracycline antibody for the tetracycline antigen binding sites. Any unbound enzyme conjugate is then removed in a washing step. Substrate solution (TMB) is added to the wells and incubated. Bound enzyme conjugate converts the colorless chromogen into a blue product. The addition of the Stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the tetracycline concentration in the sample.

3. Materials Supplied With Kit

3.1 Antigen Coated Microwell (8 wells×12 strips): precoated with tetracycline antigen.

3.2 Tetracycline standard solutions (2mL each): 0 ppb、0.5ppb、1 ppb、2 ppb、10ppb.

3.3 Tetracycline antibody:10mL

3.4 Enzyme antibody: 10mL

3.5 10×Washing buffer: 50mL

3.6 Diluent Buffer A: 50mL

3.7 Diluent Buffer B: 50mL

3.8 Diluent Buffer C: 50mL

3.9 5×Sample extract: 50mL

3.10 TMB: 17mL.

3.11 Stop solution:7mL.

3.12 Product Manual.

3.13 QC report.