C/N: EE 2013

1. BACKGROUND
Salbutamol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of salbutamol in feed, meat and urine. Salbutamol can improve the meat/fat ratio in fattened animals and accelerate livestock growth. They also have a relaxing effect on non-striated musculature and can be used as antiasthmatic and tocolytic agents in human beings. Therefore, excess salbutamol residues in livestock products may lead to health risks for consumers. This has led to a prohibition of salbutamol use in food production. The amount of salbutamol residues can be determined by methods such as HPLC or GC-MS. However, these traditional methods usually require very expensive preparation procedures. Alternatively, enzyme immunoassay (ELISA) can be used as a screening system, which is simple, rapid, sensitive and cost-effective compared with traditional methods.

2. PURPOSE
This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Salbutamol residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the clenbuterol concentration in the sample.

3. KIT CONTENTS
1)Microtiter plate (8 wells×12) precoated with antigen.
2)7×Salbutamol standard solutions (1ml/each):0ng/ml, 0.1ng/ml, 0.3ng/ml, 1.0ng/ml, 3.0ng/ml, 9.0ng/ml, 27.0ng/ml.
Controls provided: 0ppb, 0.1ppb, 0.3ppb, 1.0ppb, 3.0ppb, 9.0ppb and 27.0ppb
3)Antibody (100×) :Salbutamol (0.2ml)
4)Enzyme Conjugate(100×) (0.2ml)
5)Washing buffer(10×) (50ml)
6)Antibody and Enzyme diluent buffer (30ml)
7)Diluent buffer A (20ml)
8)Diluent buffer B (40ml)
9)Diluent buffer C (40ml)
10)TMB (18ml)
11)Stop solution (7ml)
12)Product Manual