C/N:ES 108

1. BACKGROUND

iCheck®-Fum strip is lateral flow immunochromatographic assay based on a competitive immunoassay format. With iCheck® strip reader, it measures 0-5000ppb of Fum in grain.

2. MATERIALS PROVIDED

1) iCheck®-Fum strip (25/pk).

2) Microwells coated (25)

3) Dilution buffer CSB-1(5mL)

4) 200µL Pipette Tip (50)

5) Fum QR Code

6) Product Manual

Materials required but not provided:

1) 200 and 1000µL precision micropipette

2) iCheck® Strip reader (with ZERO card)

3) Multi-purpose rotary mixer

4) Timer

5) Weigh scale

6) Distilled water or pure water

7) Extraction solution: Methanol+Water(70+30)

8) Funnel

9) Filter paper

10) 50mL Extraction tube

3. PRECAUTIONS

1) Store test kits at 2~8℃(35.6~46.4℉) when not in use, and do not use beyond the expiration date.

2) Keep from moisture, and do not use the damaged and damp kits.

3) All reagents must be at room temperature (25℃±2℃) before assay is running.

4) Do not re-use test strips.

5) Avoid swallowing the desiccant.

4. INTERPRETATION OF THE RESULTS

1) Test range: 0-5000ppb, LOQ 500ppb.

2) If there is no line in control zone, the test is invalid and the sample should be re-tested by using a valid test strip.

3) If reading is 5000 ppb, the filtered extract should be further diluted in 70% methanol such that the diluted sample reading results are in a range of 0-5000ppb and reanalyzed to obtain accurate results. The dilution factor must be included when the final result is calculated.