C/N: EE 104

1. BACKGROUND
The mycotoxin ochratoxin A is formed by fungi of the species Aspergillus and Penicillium. Apart from a marked nephrotoxicity, ochratoxin A displays hepatotoxic, teratogenic, carcinogenic and immunosuppressive properties. There is a risk to human health not only through the intake of contaminated foods of vegetable origin, but also through foods of animal origin.

2. PURPOSE
This handbook establishes procedures for determining ochratoxin A in grain and processed grain products. The test is a competitive direct ELISA that provides exact concentrations in parts per billion (ppb). Free toxin in the sample and controls competes with enzyme-labeled toxin (conjugate) for the antibody binding sites. After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color.

3. KIT CONTENTS
1)Microtiter plate (8 wells×12) precoated with antibodies to mouse IgG.
2)5×Ochratoxin A standard solutions (1ml/each):0ng/ml, 0. 1ng/ml, 0.3ng/ml, 1.0ng/ml, 2.0ng/ml
3)Controls provided: 0ppb, 0. 1ppb, 0.3ppb, 1.0ppb,and 2.0ppb
4)Antibody (100×): Ochratoxin A (0.2ml)
5)Enzyme Conjugate (100×) (0.2ml)
6)Washing buffer (10×) (50ml)
7)Antibody and Enzyme diluent buffer (30ml)
8)Diluent buffer (50ml)
9)TMB (17ml)
10)Stop solution (7ml)
11)Product Manual