C/N:EE 2042

1. BACKGROUND

Kanamycin sulfate is an aminoglycoside antibiotic produced by Streptomyces kanamyceticus. Aminoglycoside antibiotic kanamycin is being widely used in animal diseases range, because of its neurotoxicity and renal toxicity, damage to the 8th cranial nerve, resulting in vestibular and cochlear damage, mainly the proximal renal toxicity Body curved pipe damage, the protein in urine, hematuria, kidney dysfunction and so on.Kanamycin can cause severe damage to the kidneys and can also cause hearing loss. Residues in animal food may affect human health, Europe, America and China have urged their limited use. Currently, ELISA detection of kanamycin residues as a screening method has been widely used.

2. PURPOSE

This handbook establishes procedures for determining Kanamycin in grain and processed grain products. The test is a competitive direct ELISA that provides exact concentrations in parts per billion (ppb). Free toxin in the sample and controls competes with enzyme-labeled toxin (conjugate) for the antibody binding sites. After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color.

3. KIT CONTENTS

1)Microtiter plate (8 wells×12) precoated with antibodies to mouse IgG.
2)6×kanamycin standard solutions (1ml/each):0ng/ml, 1.0ng/ml, 3.0ng/ml, 8.0ng/ml, 16.0ng/ml, 32.0ng/ml
Controls provided: 0ppb, 1.0ppb, 3.0ppb, 8.0ppb, 16.0ppb and 32.0ppb
3)Kanamycin Antibody (100×) (0.2ml)
4)Enzyme Conjugate(100×) (0.2ml)
5)Washing buffer(10×) (50ml)
6)Antibody and Enzyme diluent buffer (30ml)
7)Diluent buffer (2×50ml)
8)TMB (18ml)