C/N:EE107

1. BACKGROUND

The mycotoxin T-2 is formed by beaded sickle bacteria, rotavirus sickle bacteria. T-2 producing fungi commonly attack grains and can grow at temperatures from slightly above freezing to about 86°F (30°C). All domestic animals are susceptible to injury by dietary intake of T-2 in the range of a few ppm. In poultry, feed contaminated with 1.0 to 3.5 ppm of T-2 has produced lesions at the edges of the beaks, abnormal feathering in chicks, a drastic and sudden drop in egg production, eggs with thin shells, reduced weight gains, and mortality.

2. PURPOSE

This handbook establishes procedures for determining T2 in corn, grain and feed. The test is a competitive direct ELISA that provides exact concentrations in parts per billion (ppb). Free toxin in the sample and controls competes with enzyme-labeled toxin (conjugate) for the antibody binding sites.After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color. A stop solution is then add which changes the color from blue to yellow. The intensity of the color is inversely proportional to the concentration of T2 in the samples and standards.

3. KIT CONTENTS

1) Microtiter plate ( 8 wells×12) precoated with antibodies to mouse IgG.

2) T-2 standard solutions (1mL/each): 0ng/mL、0.5ng/mL、1.0ng/mL、2.0ng/mL、5.0ng/mL、20ng/mL

3) T-2 Enzyme Antibody:(10mL)

4) T-2 Antibody : (10mL)

5) T-2 Diluent Buffer A: (50mL)

6) T-2 Diluent Buffer B: (50mL)

7) TMB:(17mL)

8) Stop solution:(7mL)

9) Product Manual

10) COA report