C/N: EE108

1. BACKGROUND

The mycotoxin Fumonisins is formed by beaded sickle bacteria, rotavirus sickle bacteria. Fumonisin-B1 and Fumonisin-B2 are two of the most common and harmful Fumonisins. Fumonisins mainly exists in corn and corn products. It has be classified into Class 2B carcinogen.

2. PURPOSE

This handbook establishes procedures for determining Fumonisins in corn, grain and feed. The test is a competitive direct ELISA that provides exact concentrations in parts per billion (ppb). Free toxin in the sample and controls competes with enzyme-labeled toxin (conjugate) for the antibody binding sites. After a wash step, substrate reacts with the bound enzyme conjugate to produce blue color. A stop solution is then add which changes the color from blue to yellow. The intensity of the color is inversely proportional to the concentration of Fumonisins in the samples and standards.

3. KIT CONTENTS

1) Microtiter plate ( 8 wells×12) precoated with antibodies to mouse IgG.

2) Fumonisins standard solutions (1mL/each):0ng/mL, 1ng/mL, 4ng/mL, 10ng/mL, 50ng/mL

3) Fum Enzyme Conjugate:(10mL)

4) Fum Antibody : (10mL)

5) Fum Diluent Buffer A: (50mL)

6) Fum Diluent Buffer B: (50mL)

7) Washing buffer (10×):(50mL)

8) TMB:(17mL)

9) Stop solution:(7mL)

10) Product Manual

11) COA report