1.0  INTENDED USE

   A simple and efficient extraction and purification procedure for 1-Aminohydantoin (AHD)was developed by means of the immunoaffinity column (IAC-AHD) as a cleanup tool．AHD content in Feeds,Aquatic Products and Animal Derived Food are cleaned up by IAC and determined by HPLC or LC/MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring AHD

2.0 PRINCIPLE

   Samples are prepared by mixing with an extraction solution, blending and filtering.  The extract is then applied to the AHD immunoaffinity column bound with specific antibodies to AHD．At this stage, the AHD binds to the antibody on the column.  The column is then washed with water to rid the immunoaffinity column of impurities.  By passing methanol through the column, the AHD is removed from the antibody.  This methanol solution can then be injected into HPLC or LC/MS/MS system.