C/N: EE 116    Sensitivity: 0.05 ppb    Test Range: 0.05~5.0 ppb

1. BACKGROUND
    Zearalenone is produced by natural fusarium, and the product of reduction of which is zeranol. Zeranol is a female hormone, which maintains secondary sexual characteristics and protein assimilation effect. It can be used for growth promotion of animals besides therapy, especially in cattle. It is now being inhibited for growth promotion in many countries for the obvious carcinogenicity.

2. PURPOSE
   This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Zeranol residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the clenbuterol concentration in the sample.

3. KIT CONTENTS
1)Microtiter plate (8 wells×12) precoated with antigen.
2)7×Zeranol standard solutions (1ml/each):0ng/ml, 0.05ng/ml, 0.2ng/ml, 0.5ng/ml, 1.0ng/ml, 2.0ng/ml, 5.0ng/ml
Controls provided: 0ppb, 0.05ppb, 0.2ppb, 0.5ppb, 1.0ppb, 2.0ng/ml and 5.0ppb
3)Antiboby (200×) :Zeranol           (0.2ml)
4)Enzyme Conjugate(100×)           (0.2ml)
5)Washing buffer (10×)               (50ml)
6)Antibody and Enzyme diluent buffer   (30ml)
7)Diluent buffer A                   (50ml)
8)Diluent buffer B                   (50ml)
9)Diluent buffer C                   (50ml)
10)Diluent buffer D                   (30ml)
11)TMB                                (13ml)
12)Stop solution                      (7ml)
13)Product Manual